Zip file attached is the other thing where if someone tries to use it. The L929-bGH cells used in this study also retained the ligand

When this cell line was transfected with the bGH construct, the serum levels of bGH could be significantly increased by the presence of the transgene. The transgene expression was also tested in vitro using a bGH-responsive cell line, the L929-bGH cells. The age-related increase in the serum levels of bGH correlated to a decrease in the bGH levels in the brain of 2-month-old transgenic mice.

In transgenic mice that were about 1 yr old, the highest growth response was found in the liver and testes, but only low levels were seen in the brain. The highest levels of bGH were found in the serum, liver, testes, and in muscle tissues of 2-month-old transgenic mice, while in the brain no bGH could be detected. The transgenic mice exhibited a high, specific growth response in vivo. The transgenic mice were screened for the presence of the bGH cDNA by polymerase chain reaction and verified for the presence of bGH by means of immunohistochemistry.

For this purpose, a construct containing the bGH cDNA sequence was used, and the cDNA fragment was microinjected into fertilized eggs of CD-1 mice. The development of a new transgenic mouse line was undertaken in order to study tissue-specific expression of bovine growth hormone (bGH) in vitro and in vivo. Tissue-specific expression of bovine GH in liver and muscle cells of transgenic mice. I don’t think what you need to do would be anything as hard as that and as hard as it is to do, you may be able to get the results that you’re looking for if you try that method, maybe with a little more searching on the web. that can be adapted to the specific needs of the user.

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