However, G2A activation was not sufficient to promote M1-polarization in bone marrow-derived macrophages. Fittingly, the number of M1-like macrophages was reduced in the inflamed tissue in G2A-deficient mice.

G2A-deficient mice showed a reduced zymosan-induced thermal hyperalgesia, which was reversed after macrophage depletion.

Here, we studied the role of the G-protein coupled receptor G2A (GPR132), in chemotactic migration and polarization of macrophages, using the zymosan-model of acute inflammation. Macrophages are highly versatile cells, which acquire, depending on their microenvironment, pro- (M1-like), or antiinflammatory (M2-like) phenotypes.

3Faculty of Medicine, Institute of Biochemistry I, Goethe-University Frankfurt, Frankfurt, Germany.

2Project Group Translational Medicine and Pharmacology, Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Frankfurt, Germany.

1Institute of Clinical Pharmacology, Pharmazentrum Frankfurt, University Hospital Frankfurt, Frankfurt, Germany.

Schäfer 1 †, Jennifer Cohnen 1, Sandra Pierre 1, Tabea Osthues 2, Neda Tarighi 1, Stefan Hohmann 1, Nerea Ferreiros 1, Bernhard Brüne 2,3, Andreas Weigert 3, Gerd Geisslinger 1,2, Marco Sisignano 1 and Klaus Scholich 1,2 *

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